Marino M, Spagnuolo S, Cavallini M, Terenzi F, Mangiantini M T, Leoni S
Dipartimento di Biologia Cellulare e dello Sviluppo, Università La Sapienza, Roma, Italy.
Cell Signal. 1996 Dec;8(8):555-9. doi: 10.1016/s0898-6568(96)00111-8.
We investigate the effect of EGF on IP3 production, PLC gamma phosphorylation, calcium transients in rat hepatocytes isolated in quiescent liver (G0 phase of cell cycle) and at 4 h (G1 phase of cell cycle) and 24 h (M phase of cell cycle) after partial hepatectomy. Our results show that EGF does not utilize IP3 and calcium as its signal transduction molecules when the hepatocytes are in vivo stimulated to entry in the cell cycle. In particular the growth factor does not phosphorylate PLC gamma and induces a decrease in IP3 content. These data suggest that EGF utilizes different signal transduction to send information from receptor to nucleus during PH with respect to the quiescent liver.
我们研究了表皮生长因子（EGF）对在静止肝脏（细胞周期的G0期）中分离的大鼠肝细胞以及在部分肝切除术后4小时（细胞周期的G1期）和24小时（细胞周期的M期）时的肌醇三磷酸（IP3）生成、磷脂酶Cγ（PLCγ）磷酸化和钙瞬变的影响。我们的结果表明，当肝细胞在体内被刺激进入细胞周期时，EGF并不利用IP3和钙作为其信号转导分子。特别是，该生长因子不会使PLCγ磷酸化，并导致IP3含量降低。这些数据表明，与静止肝脏相比，EGF在部分肝切除期间利用不同的信号转导将信息从受体传递至细胞核。
