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大鼠卵黄囊和肝癌的甲胎蛋白信使核糖核酸

alpha 1-Fetoprotein mRNA of rat yolk sac and hepatoma.

作者信息

Chiu J F, Decha-Umphai W, Commer P

出版信息

Nucleic Acids Res. 1979 Sep 11;7(1):239-49. doi: 10.1093/nar/7.1.239.

DOI:10.1093/nar/7.1.239
PMID:91159
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328009/
Abstract

Rat alpha 1-fetoprotein mRNA was isolated and purified to apparent homogeneity by means of immunoadsorption and oligo (dT) cellulose affinity chromatography. Purified AFP mRNA migrated as a 21S peak in 2.5% SDS-polyacrylamide gels. The translation product of this mRNA in micrococcal nuclease treated reticulocyte lysate was identified as AFP by specific immunoprecipitation, SDS-gel electrophoresis and tryptic digestion analysis. DNA complimentary to AFP mRNA was synthesized with avian meyloblastosis virus RNA-dependent DNA polymerase. This AFP cDNA was used as a probe to quantitate AFP mRNA in the developing rat liver and to compare the complexity and diversity of AFP mRNA derived from the normal rat liver and Morris hepatoma 7777. We found that the amount of functional AFP mRNA is decreasing during liver development. There is very little, if any, AFP mRNA in the adult rat liver. A high degree of homology between the AFP mRNA sequences of yolk sac and hepatoma was also found.

摘要

通过免疫吸附和寡聚（dT）纤维素亲和层析法，分离并纯化大鼠α1-甲胎蛋白信使核糖核酸（mRNA），使其达到明显的均一性。纯化后的甲胎蛋白信使核糖核酸在2.5%十二烷基硫酸钠-聚丙烯酰胺凝胶中迁移为一个21S峰。通过特异性免疫沉淀、十二烷基硫酸钠-凝胶电泳和胰蛋白酶消化分析，确定该信使核糖核酸在微球菌核酸酶处理的网织红细胞裂解物中的翻译产物为甲胎蛋白。用禽成髓细胞瘤病毒RNA依赖性DNA聚合酶合成与甲胎蛋白信使核糖核酸互补的DNA。该甲胎蛋白互补脱氧核糖核酸用作探针，以定量发育中大鼠肝脏中的甲胎蛋白信使核糖核酸，并比较正常大鼠肝脏和莫里斯肝癌7777来源的甲胎蛋白信使核糖核酸的复杂性和多样性。我们发现，在肝脏发育过程中，功能性甲胎蛋白信使核糖核酸的量在减少。成年大鼠肝脏中即使有甲胎蛋白信使核糖核酸，也非常少。还发现卵黄囊和肝癌的甲胎蛋白信使核糖核酸序列之间有高度同源性。

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本文引用的文献

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Production of embryonal alpha-globulin by transplantable mouse hepatomas.可移植性小鼠肝癌产生胚胎性α-球蛋白
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Sites of serum alpha-fetoprotein synthesis in the human and in the rat.人类和大鼠血清甲胎蛋白的合成部位
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Biochemistry. 1974 Aug 13;13(17):3606-15. doi: 10.1021/bi00714a032.
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Specific binding of albumin antibody to rat liver polysomes.白蛋白抗体与大鼠肝脏多核糖体的特异性结合。
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Immunoadsorption of ovalbumin synthesizing polysosmes and partial purification of ovalbumin messenger RNA.卵清蛋白合成多核糖体的免疫吸附及卵清蛋白信使核糖核酸的部分纯化
Methods Enzymol. 1974;30:631-48. doi: 10.1016/0076-6879(74)30061-4.
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Use of antibodies for the isolation of biologically pure messenger ribonucleic acid from fully functional eukaryotic cells.使用抗体从功能完整的真核细胞中分离生物纯信使核糖核酸。
Biochemistry. 1974 Apr 23;13(9):1875-85. doi: 10.1021/bi00706a016.
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Rat alpha-fetoprotein. 8. Refinement of radioimmunoassay for detection of 1 ng rat alpha 1F.大鼠甲胎蛋白。8. 用于检测1纳克大鼠甲胎蛋白的放射免疫测定法的改进。
Immunochemistry. 1973 Jul;10(7):439-42. doi: 10.1016/0019-2791(73)90013-x.
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Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.
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Relationship of rat alpha 1-fetoprotein to growth rate and chromosome composition of Morris hepatomas.大鼠甲胎蛋白与莫里斯肝癌生长速率及染色体组成的关系。
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