Woloschak G E, Schreck S, Panozzo J, Chang-Liu C M, Libertin C R
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, IL 60439-4833, USA.
Biochim Biophys Acta. 1997 Mar 20;1351(1-2):105-10. doi: 10.1016/s0167-4781(96)00184-4.
Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. gamma rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that gamma-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. Doses which caused over 99% cell killing induced HIV-LTR transcription maximally, demonstrating that cells that will go on to die by 14 days are the cells expressing HIV-LTR-CAT.
利用稳定转染了HIV-LTR-CAT构建体的HeLa细胞，我们证明了在暴露于某些细胞杀伤剂24小时后，CAT诱导出现峰值，该峰值出现在存活（但不一定具有细胞分裂能力）的细胞中。γ射线是唯一不诱导HIV转录的细胞杀伤剂；这可归因于γ射线诱导的凋亡性死亡需要功能性p53，而HeLa细胞中不存在这种蛋白。对于所有其他试剂，HIV-LTR诱导呈剂量依赖性，且与培养物中发生的细胞杀伤量相关。导致超过99%细胞死亡的剂量可最大程度地诱导HIV-LTR转录，这表明在14天内会死亡的细胞就是表达HIV-LTR-CAT的细胞。
