Caporale D A, Beal B F, Roxby R, Van Beneden R J
Department of Zoology, University of Maine, Orono 04469, USA.
Mol Mar Biol Biotechnol. 1997 Mar;6(1):33-9.
Sequences of the internal transcribed spacer (ITS-1) ribosomal DNA region were compared among 88 soft-shell clams (Mya arenaria) from 12 sites (within three general areas) along the New England coast to determine whether populations were genetically heterogeneous. Two sequence variants were observed, with type 1 having a 3-nucleotide insertion and one point mutation relative to type 2. Allele-specific polymerase chain reaction (PCR); using primers specific to each sequence type, was performed to determine the distribution of individuals who had both allelic forms. DNA from soft-shell clams collected from three areas (Cobscook Bay, Maine; Gulf of Maine; and southern New England) were compared chi 2 analyses of allele-specific PCR results revealed no significant heterogeneity among the three population distributions.
对来自新英格兰海岸12个地点（分属三个大致区域）的88只软壳蛤（砂海螂）的核糖体DNA内部转录间隔区（ITS-1）序列进行了比较，以确定种群在基因上是否存在异质性。观察到两个序列变体，相对于2型，1型有一个3核苷酸插入和一个点突变。使用针对每种序列类型的特异性引物进行等位基因特异性聚合酶链反应（PCR），以确定具有两种等位基因形式的个体的分布情况。对从三个区域（缅因州的科布斯科克湾、缅因湾和新英格兰南部）采集的软壳蛤的DNA进行了比较，等位基因特异性PCR结果的卡方分析显示，这三个种群分布之间没有显著的异质性。
