特应性皮炎患者中针对卵圆形糠秕孢子菌蛋白质和甘露聚糖抗原的IgE抗体。

IgE antibodies to protein and mannan antigens of pityrosporum ovale in atopic dermatitis patients.

作者信息

Lintu P, Savolainen J, Kalimo K

机构信息

Department of Clinical Allergology, University of Turku, Finland.

出版信息

Clin Exp Allergy. 1997 Jan;27(1):87-95.

PMID:9117887

Abstract

BACKGROUND

Pityrosporum ovale is a common saprophyte on the skin capable of inducing IgE antibody production in atopic dermatitis (AD) patients. Allergens of P. ovale have been examined in several studies, but consensus on them is lacking.

OBJECTIVE

This study was carried out to obtain more information about the IgE antibody response against P. ovale, including mannan.

METHODS

Sera from 64 AD patients and 10 healthy controls were analysed with immunoblotting and the nitrocellulose radio allergosorbent test (RAST) method specifically developed to detect antimannan P. ovale IgE antibodies.

RESULTS

In immunoblotting a total of 39 different IgE stained protein bands were seen. A high molecular weight staining was also seen especially in patients who displayed elevated mannan P. ovale RAST values. The most commonly stained protein bands in immunoblotting were 9 and 96 kD bands with antibodies in 73 and 65% of AD patients who had been positive in commercial P. orbiculare RAST with total serum IgE less than 4000 kU/l. Mannan RAST appeared positive in 77% of them. Positive immunoblotting to either of these bands was seen in 90% and, if added with staining with the 20 kD band, in 100% of these AD patients. A combination of 9 kD IgE staining and mannan P. ovale RAST was positive in 92% of the patients and 96 kD and mannan P. ovale RAST in 85% of the patients.

CONCLUSION

It is evident that P. ovale has several allergens, the 9, 96 and 20 kD regions being the most important. According to our results mannan is also an important allegen of P. ovale.

摘要

背景

卵圆形糠秕孢子菌是皮肤上常见的腐生菌，能够在特应性皮炎（AD）患者中诱导IgE抗体产生。多项研究已对卵圆形糠秕孢子菌的变应原进行了检测，但尚未达成共识。

目的

本研究旨在获取更多关于针对卵圆形糠秕孢子菌（包括甘露聚糖）的IgE抗体反应的信息。

方法

采用免疫印迹法以及专门开发用于检测抗卵圆形糠秕孢子菌甘露聚糖IgE抗体的硝酸纤维素放射变应原吸附试验（RAST）方法，对64例AD患者和10名健康对照者的血清进行分析。

结果

在免疫印迹中，共观察到39条不同的IgE染色蛋白条带。还观察到一条高分子量条带，尤其在卵圆形糠秕孢子菌甘露聚糖RAST值升高的患者中。免疫印迹中最常染色的蛋白条带是9 kD和96 kD条带，在总血清IgE低于4000 kU/l且商业圆形糠秕孢子菌RAST呈阳性的AD患者中，分别有73%和65%的患者抗体呈阳性。77%的患者甘露聚糖RAST呈阳性。这些AD患者中，90%的患者对这两条带中的任何一条免疫印迹呈阳性，若加上20 kD条带染色，则100%呈阳性。9 kD IgE染色与卵圆形糠秕孢子菌甘露聚糖RAST联合检测，92%的患者呈阳性；96 kD与卵圆形糠秕孢子菌甘露聚糖RAST联合检测，85%的患者呈阳性。