Kaluz S, Kaluzova M, Flint A P
Department of Physiology and Environmental Science, University of Nottingham, Loughborough,
Genet Anal. 1996 Dec;13(6):165-9. doi: 10.1016/s1050-3862(96)00168-4.
Combination of suppression polymerase chain reaction (PCR) and megaprimer method of site-directed mutagenesis allows specific reamplification of extended megaprimer. This modification is proposed for templates that are difficult to amplify when standard megaprimer procedures do not generate sufficient yield of mutated product.
