Iwasaki K, Sunderland T, Kusiak J W, Wolozin B
Section on Geriatric Psychiatry, National Institute of Mental Health, Bethesda, MD 20892-1264, USA.
Mol Psychiatry. 1996 Mar;1(1):65-71.
The a beta peptide induces cell death in neurons grown in cell culture. Previous studies have shown that the mechanism of a beta-mediated cell death of central nervous system neurons appears to be via apoptosis. Apoptosis is an active process that involves both gene transcription and translation. Using semi-quantitative polymerase chain reaction, we have analyzed the levels of a variety of transcripts in primary neuronal cultures treated with a beta that are likely to play important roles in apoptosis. Following addition of 10 microM a beta 1-42 the immediate early response gene, c-fos, shows a rapid and sustained increase in transcript level while c-jun levels increase at a slower rate. Bcl-2 and its homologues, bcl-X and bax, also increase in amount with bcl-2 and bcl-X increasing more rapidly than bax. These data provide support indicating that a beta-mediated cell death in central nervous system neurons is an active process similar to that seen in apoptosis.
β-淀粉样肽可诱导细胞培养中生长的神经元死亡。先前的研究表明，β-淀粉样肽介导的中枢神经系统神经元细胞死亡机制似乎是通过凋亡实现的。凋亡是一个涉及基因转录和翻译的活跃过程。我们使用半定量聚合酶链反应，分析了用β-淀粉样肽处理的原代神经元培养物中各种转录本的水平，这些转录本可能在凋亡中发挥重要作用。加入10微摩尔的β-淀粉样肽1-42后，即时早期反应基因c-fos的转录水平迅速且持续增加，而c-jun水平以较慢的速度增加。Bcl-2及其同源物bcl-X和bax的量也增加，其中bcl-2和bcl-X的增加速度比bax更快。这些数据支持了这样一种观点，即中枢神经系统神经元中β-淀粉样肽介导的细胞死亡是一个类似于凋亡的活跃过程。
