Holzmann K, Korosec T, Gerner C, Grimm R, Sauermann G
Institute of Tumor Biology-Cancer Research, University of Vienna, Austria.
Eur J Biochem. 1997 Mar 1;244(2):479-86. doi: 10.1111/j.1432-1033.1997.00479.x.
Nuclear-matrix proteins were prepared from different rat and human cells and separated by two-dimensional gel electrophoresis. By computer-assisted analysis of the images, two of the proteins were identified as ubiquitously occurring (common) nuclear-matrix proteins, which appeared in tissue-dependent concentrations. The two proteins that originated from human blood mononuclear cells were analyzed further. Tryptic digests of the blotted proteins were analyzed by partial peptide sequencing and matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry. The two human common nuclear-matrix proteins were identified as heterogeneous nuclear ribonucleoproteins (hnRNP) H and H' or their variants. Furthermore, mass analysis revealed details on the N terminus of hnRNP H.
从不同的大鼠和人类细胞中制备核基质蛋白，并通过二维凝胶电泳进行分离。通过对图像的计算机辅助分析，鉴定出其中两种蛋白质为普遍存在（常见）的核基质蛋白，其浓度呈现组织依赖性。对源自人类血液单核细胞的这两种蛋白质进行了进一步分析。通过部分肽测序和基质辅助激光解吸电离飞行时间质谱对印迹蛋白质的胰蛋白酶消化产物进行分析。这两种人类常见核基质蛋白被鉴定为不均一核核糖核蛋白（hnRNP）H和H'或其变体。此外，质谱分析揭示了hnRNP H N端的详细信息。
