[Characterization of protein composition of guinea pig skin melanosomes by polyacrylamide gel electrophoresis].

Protein composition and molecular weights of guinea pig skin melanosoma proteins were determined by polyacrylamide gel electrophoresis. Treatment of melanosomas extracts with sodium dodecyl sulfate, Triton X-100 and 1 N NaOH followed by electrophoresis revealed 26, 15 and 8-10 protein zones, respectively. It was found that melanosomas contain 4 main protein groups with respective molecular weights of 10 000-13 000, 21 000-42 000-94 000 and over 100 000.