Solovev A A, Churunov V A, Erokhin Iu E
Biokhimiia. 1977 Sep;42(9):1697-703.
A subchromatophore fraction containing the reaction center P-840 was isolated from the chromatophores of green serobacteria Chlorobium limicola forma thiosulfatophilum by ultracentrifugation and its protein composition was characterized. After treatment of the chromatophores by Triton X-100 and subsequent polyacrylamide gel electrophoresis two pigment-protein complex containing bacterioviridin. The proteins of the pigment-protein complex containing bacterioviridin. The proteins of the pigment-protein complexes were obtained. The first complex contained mainly bacteriochlorophyll alpha with bacterioviridin contaminations and the second one-only bacterioviridin. The low-temperature absorption spectrum of the bacteriochlorophyll alpha-containing pigment-protein complex is identical to the absorption spectrum of a water-soluble pigment-protein complex isolated and characterized by Olson and contains proteins with molecular weights of 34.800, 33.100, 27.500 and 23.200. A protein with molecular weight of 32.700 was found in the pigment-protein complexes were compared to the proteins of the photochemically active subchromatophore fraction and chromatophores.
通过超速离心从嗜硫绿菌属绿硫细菌（Chlorobium limicola forma thiosulfatophilum）的载色体中分离出含有反应中心P - 840的亚载色体部分，并对其蛋白质组成进行了表征。用Triton X - 100处理载色体并随后进行聚丙烯酰胺凝胶电泳后，得到了两种含绿菌绿素的色素 - 蛋白质复合物。得到了含绿菌绿素的色素 - 蛋白质复合物的蛋白质。第一种复合物主要含有被绿菌绿素污染的细菌叶绿素α，第二种仅含有绿菌绿素。含细菌叶绿素α的色素 - 蛋白质复合物的低温吸收光谱与奥尔森分离并表征的水溶性色素 - 蛋白质复合物的吸收光谱相同，且含有分子量为34800、33100、27500和23200的蛋白质。在色素 - 蛋白质复合物中发现了一种分子量为32700的蛋白质，并将这些色素 - 蛋白质复合物的蛋白质与光化学活性亚载色体部分和载色体的蛋白质进行了比较。
