Graus F, Dalmau J, Valldeoriola F, Ferrer I, Reñe R, Marin C, Vecht C J, Arbizu T, Targa C, Moll J W
Service of Neurology, Hospital Clínic i Provincial, Barcelona, Spain.
J Neuroimmunol. 1997 Apr;74(1-2):55-61. doi: 10.1016/s0165-5728(96)00205-6.
We studied an autoantibody (called anti-Tr), found in the serum and CSF of five patients with paraneoplastic cerebellar degeneration (PCD) and Hodgkin's disease (HD). Anti-Tr antibodies labelled the cytoplasm of Purkinje cells of human and rat cerebellum. The molecular layer of rat cerebellum showed a characteristic dotted pattern suggestive of immunoreactivity of dendritic spines of Purkinje cells. Patients with cerebellar disorders without HD (159) or HD without PCD (30) did not harbor anti-Tr antibodies. Immunoblots of human Purkinje cells or rat and mouse cerebellum were negative. Anti-Tr antibodies, as defined in this study, appear specific for HD-associated PCD. The immunohistochemical pattern described in the rat cerebellum coupled with the absence of reactivity in the immunoblot may be used to identify anti-Tr antibodies.
我们研究了一种自身抗体（称为抗Tr），在5例副肿瘤性小脑变性（PCD）合并霍奇金病（HD）患者的血清和脑脊液中发现了该抗体。抗Tr抗体标记了人和大鼠小脑浦肯野细胞的细胞质。大鼠小脑分子层呈现出特征性的点状模式，提示浦肯野细胞树突棘具有免疫反应性。无HD的小脑疾病患者（159例）或无PCD的HD患者（30例）未检测到抗Tr抗体。人浦肯野细胞或大鼠及小鼠小脑的免疫印迹均为阴性。本研究中定义的抗Tr抗体似乎对HD相关的PCD具有特异性。大鼠小脑中描述的免疫组织化学模式以及免疫印迹中无反应性可用于鉴定抗Tr抗体。
