Barrington G M, Besser T E, Gay C C, Davis W C, Reeves J J, McFadden T B
Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USA.
J Dairy Sci. 1997 Jan;80(1):94-100. doi: 10.3168/jds.S0022-0302(97)75916-2.
Explants of mammary tissue from cows in late pregnancy were incubated for 72 h in serum-free, hormonally defined media to investigate the regulation of the bovine mammary IgG1 receptor. Treatments included incubation in basal medium alone, basal medium plus estradiol-17 beta, basal medium plus prolactin, or basal medium plus estradiol-17 beta and prolactin. alpha-Lactalbumin production was measured by radioimmunoassay in culture supernatants collected at 24, 48, and 72 h. Explants were examined immunohistochemically for expression of the IgG1 receptor at 24, 48, and 72 h. alpha-Lactalbumin concentrations increased, and IgG1 receptor expression decreased, by 72 h with explants cultured in the medium containing prolactin. Results suggest that, in addition to its positive lactogenic effect, prolactin decreases expression of the bovine mammary IgG1 receptor.
将妊娠后期奶牛的乳腺组织外植体在无血清、激素明确的培养基中孵育72小时，以研究牛乳腺IgG1受体的调节。处理包括仅在基础培养基中孵育、基础培养基加雌二醇-17β、基础培养基加催乳素，或基础培养基加雌二醇-17β和催乳素。在24、48和72小时收集的培养上清液中通过放射免疫测定法测量α-乳白蛋白的产生。在24、48和72小时对外植体进行免疫组织化学检查以检测IgG1受体的表达。在含有催乳素的培养基中培养的外植体，到72小时时α-乳白蛋白浓度增加，而IgG1受体表达降低。结果表明，除了其积极的生乳作用外，催乳素还会降低牛乳腺IgG1受体的表达。
