Loots G P, Loots J M, Brown J M, Schoeman J L
Stain Technol. 1979 Mar;54(2):97-100. doi: 10.3109/10520297909112642.
A rapid, reliable silver impregnation method is described for nervous tissue fixed in formol-saline, Bouin or Susa. Sections are impregnated for 10-15 minutes at room temperature or 37 C in a solution containing 0.5 g Protargol-S, 0.005-0.01 g allantoin, 1 ml of 1% Cu[NO3]2, 1 ml of 1% AgNO3, and 1-2 drops of 30% H2O2 in 100 ml distilled water. Thereafter the sections are reduced in a hydroquinone-formalin solution. This is followed by gold toning and subsequent reduction and mounting. Alternatively, following the first reduction, the silver image can be intensified by placing sections in a silver-allantoin bath which is followed by reduction and mounting. This method is very reliable and selective, making it suitable for general routine and research use.
本文描述了一种快速、可靠的银浸染方法，用于处理用甲醛生理盐水、布因液或苏萨液固定的神经组织。切片在室温或37℃下，于含有0.5g普罗他哥尔-S、0.005 - 0.01g尿囊素、1ml 1%硝酸铜、1ml 1%硝酸银以及100ml蒸馏水中1 - 2滴30%过氧化氢的溶液中浸染10 - 15分钟。之后，切片在对苯二酚 - 甲醛溶液中进行还原。接着进行金调色，随后再进行还原和封片。或者，在第一次还原后，可将切片置于银 - 尿囊素浴中强化银影像，然后进行还原和封片。该方法非常可靠且具有选择性，适用于一般常规和研究用途。
