Matthieu J M, Webster H D, Beny M, Dolivo M
Brain Res Bull. 1977 Jul-Aug;2(4):289-98. doi: 10.1016/0361-9230(77)90085-5.
Myelin and a heavy membrane fraction (1.0/1.2 fraction) were isolated from rabbit white matter by a slight modification of the procedure for bovine CNS. The specific activities of acetylcholinesterase and Na+, K+-ATPase were higher in the 1.0/1.2 fraction than in myelin. In contrast, the cerebroside content and 2'3'-cyclic nucleotide 3'-phosphohydrolase activity in the 1.0/1.2 fraction were 4.5 and 3.4 times lower than in myelin. Total lipids accounted for only 30% of the 1.0/1.2 fracton's dry weight; for myelin, they represented 70%. Polacrylamide gel electrophoresis showed the presence of many high molecular weight proteins and glycoproteins in the 1.0/1.2 fraction but myelin components were practically missing. Cytochrome c oxidase and NADPH-cytochrome c reductase activities suggested about 15% contamination in the 1.0/1.2 fraction but less than 5% for myelin. In electron micrographs of the 1.0/1.2 fraction, there were many membraneous profiles that varied in size, some mitochondrial fragments, and only a few lamellar whorls of compact myelin. The results suggest that the 1.0/1.2 fraction is different from other myelin-related fractions and is probably enriched in axolemma.
通过对牛中枢神经系统分离方法稍作修改，从兔白质中分离出髓磷脂和重膜组分（1.0/1.2组分）。乙酰胆碱酯酶和Na⁺、K⁺ - ATP酶的比活性在1.0/1.2组分中高于髓磷脂。相反，1.0/1.2组分中的脑苷脂含量和2'3'-环核苷酸3'-磷酸水解酶活性比髓磷脂低4.5倍和3.4倍。总脂质仅占1.0/1.2组分干重的30%；而髓磷脂中总脂质占70%。聚丙烯酰胺凝胶电泳显示1.0/1.2组分中存在许多高分子量蛋白质和糖蛋白，但几乎没有髓磷脂成分。细胞色素c氧化酶和NADPH - 细胞色素c还原酶活性表明1.0/1.2组分中有约15%的污染，但髓磷脂的污染小于5%。在1.0/1.2组分的电子显微照片中，有许多大小各异的膜性结构、一些线粒体片段，以及仅少数紧密髓磷脂的板层状螺旋结构。结果表明，1.0/1.2组分不同于其他与髓磷脂相关的组分，可能富含轴膜。
