Ota K, Katafuchi T, Takaki A, Hori T
Department of Physiology, Kyushu University Faculty of Medicine, Fukuoka, Japan.
Am J Physiol. 1997 Feb;272(2 Pt 2):R532-40. doi: 10.1152/ajpregu.1997.272.2.R532.
The single neuron activity in the anteroventral region of the third ventricle (AV3V) was extracellularly recorded in urethan and alpha-chloralose anesthetized rats. Electrical stimulation of the medial preoptic area (mPOA) and the paraventricular nucleus (PVN) revealed a reciprocal neural connection between the AV3V and these hypothalamic nuclei with an ipsilateral preponderance. All the AV3V neurons, which were antidromically activated by the stimulation of the mPOA or the PVN, altered their activity after the systemic injection of interleukin (IL)-1beta. On the other hand, only about 60% of the AV3V neurons that showed orthodromic responses were affected by IL-1beta. In seven of nine AV3V neurons that were electrophysiologically identified to send their axons to the mPOA or the PVN, the recombinant human IL-1beta-induced excitation and inhibition were attenuated by a local application of sodium salicylate through multibarreled micropipettes. These results suggest that the AV3V neurons alter their activity in response to the blood-borne IL-1beta, at least in part, through a local synthesis of prostanoids and then send the information to the mPOA and PVN.
在乌拉坦和α-氯醛糖麻醉的大鼠中，细胞外记录第三脑室前腹侧区域（AV3V）的单神经元活动。对内侧视前区（mPOA）和室旁核（PVN）的电刺激揭示了AV3V与这些下丘脑核之间存在相互的神经连接，且以同侧为主。所有被mPOA或PVN刺激逆向激活的AV3V神经元，在全身注射白细胞介素（IL）-1β后其活动发生改变。另一方面，仅约60%显示顺向反应的AV3V神经元受IL-1β影响。在通过电生理鉴定其轴突投射至mPOA或PVN的9个AV3V神经元中的7个中，通过多管微电极局部应用水杨酸钠可减弱重组人IL-1β诱导的兴奋和抑制。这些结果表明，AV3V神经元至少部分地通过局部合成前列腺素对血源性IL-1β作出反应，改变其活动，然后将信息传递至mPOA和PVN。
