Effects of ADP, DTT, and Mg2+ on the ion-conductive property of chloroplast H+-ATPase(CF0-CF1) reconstituted into bilayer membrane.

The purified CF0-CF1 complex of spinach was incorporated into planar lipid bilayer membranes (LBMs) formed with soybean phospholipid, and the transmembrane ion-transmission properties were studied under voltage-clamp mode. The results showed that the presence of both ADP and Pi decreased the membrane current while Dithiothreitol could evoke a stronger conductive change of CF0-CF1 containing LBMs when Ca2+ or Mg2+ exists. Mg2+ can dramatically increase the CF0-CF1 conductance in various conditions. These results indicated that the H(+)-transitive function of CF0-CF1 reconstituted in bilayer is sensitive to those factors which can affect its ATP synthase activity in vivo.