Zhang Q, Ahuja H S, Zakeri Z F, Wolgemuth D J
Department of Genetics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Dev Biol. 1997 Mar 15;183(2):222-33. doi: 10.1006/dbio.1996.8494.
In a series of studies to more precisely localize the cellular sites of expression of the cyclin-dependent kinase (Cdk) family members in reproductive organs, we observed a striking expression of Cdk5 in atretic follicles in the ovary, particularly in granulosa cells that appeared to be dying. We determined that these granulosa cells were undergoing apoptotic cell death using the in situ DNA fragmentation assay. To extend the generality of the association of Cdk5 with apoptotic cells, we examined its expression as it correlated with the detection of apoptosis in a number of developmental paradigms, including regions of the embryonic nervous system, the developing eye, and the developing limb. Finally, the association of apoptosis and Cdk5 expression and associated kinase activity was examined in the limb and in an induced cell death system, that of androgen withdrawal-induced regression of the prostate gland in male mice. These observations provide new insight into the possible function of this novel Cdk during both differentiation and apoptotic cell death.
在一系列旨在更精确地定位细胞周期蛋白依赖性激酶（Cdk）家族成员在生殖器官中表达的细胞位点的研究中，我们观察到Cdk5在卵巢闭锁卵泡中显著表达，尤其是在似乎正在死亡的颗粒细胞中。我们使用原位DNA片段化分析确定这些颗粒细胞正在经历凋亡性细胞死亡。为了扩展Cdk5与凋亡细胞关联的普遍性，我们在许多发育模式中检查了其表达，这些模式包括胚胎神经系统区域、发育中的眼睛和发育中的肢体，这些模式中均检测到了凋亡。最后，在肢体以及一个诱导性细胞死亡系统（即雄性小鼠中雄激素撤退诱导的前列腺退化）中研究了凋亡与Cdk5表达及相关激酶活性的关联。这些观察结果为这种新型Cdk在分化和凋亡性细胞死亡过程中的可能功能提供了新的见解。
