一个在转基因小鼠中指导CD8亚群特异性表达的CD8基因组片段。
A CD8 genomic fragment that directs subset-specific expression of CD8 in transgenic mice.
作者信息
Hostert A, Tolaini M, Festenstein R, McNeill L, Malissen B, Williams O, Zamoyska R, Kioussis D
机构信息
Laboratory of Molecular Immunology, National Institute for Medical Research, London, United Kingdom.
出版信息
J Immunol. 1997 May 1;158(9):4270-81.
Helper and cytotoxic T cell subsets require the expression of different coreceptors (CD4 and CD8, respectively) for their development and function. We have cloned the CD8 gene locus from genomic cosmid and P1 libraries and analyzed the region around the CD8alpha and CD8beta genes for gene expression regulatory elements. DNase I (DNase I) hypersensitivity analysis of 80 kb in the CD8 locus identified four clusters of putative regulatory regions, three of which are thymocyte specific. Transgenic mice carrying the cloned CD8alphabeta genomic locus and containing the identified DNase I-hypersensitive site clusters express the transgenic CD8 in a developmentally regulated, tissue-specific, and CD8 T cell subset-specific manner.
辅助性T细胞和细胞毒性T细胞亚群在发育和功能上需要表达不同的共受体（分别为CD4和CD8）。我们从基因组黏粒文库和P1文库中克隆了CD8基因座，并分析了CD8α和CD8β基因周围区域的基因表达调控元件。对CD8基因座中80 kb的区域进行DNA酶I（DNase I）超敏分析，确定了四个推定调控区域簇，其中三个是胸腺细胞特异性的。携带克隆的CD8αβ基因组基因座并包含已鉴定的DNase I超敏位点簇的转基因小鼠，以发育调控、组织特异性和CD8 T细胞亚群特异性的方式表达转基因CD8。
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