Konopka M, Trzciński K, Tyski S, Hryniewicz W
Sera and Vaccines Central Research Laboratory, Warsaw, Poland.
Acta Microbiol Pol. 1996;45(3-4):269-78.
One hundred and fifty hospital Staphylococcus aureus strains isolated on Polish hospitals during an outbreaks were used to evaluate usefulness of S. aureus phenotyping methods. According to the expression of resistance to methicillin (a marker of resistance to all beta-lactam antibiotics) all strains were classified as homogeneously of heterogeneously resistant to methicillin (methicillin resistant S. aureus--MRSA) and as susceptible to this antibiotic (methicillin-susceptible S. aureus--MSSA). All strains were analysed according to the resistance patterns to fifteen antistaphylococcal drugs, results of crystal violet test, phage patterns and results of biochemical fingerprinting by PhenePlate (PhP) System (BioSys inova). All MSSA analysed were resistant to penicillin and 22% to tetracycline, and only occasionally resistant to other antimicrobials. They showed sensitive to phages of all international and additional lytic groups. Heterogeneous MRSA were widely susceptible to almost all antimicrobials except tetracycline and were lysed by phages of Ist, IIIrd international groups and additional phages. All these strains produced penicillinases. Homogeneous MRSA were multi-drug resistant and were not typable by phages of Ist and IInd lytic groups and weakly typable by phages of IIIrd and additional groups. PhP Systems divided all used strains into 13 common (with two dominating) and 50 single strain PhP-types. The combination of various phenotyping methods may be useful in epidemiological investigation of hospitals.
在波兰医院的一次暴发期间分离出的150株医院金黄色葡萄球菌菌株用于评估金黄色葡萄球菌表型分析方法的实用性。根据对甲氧西林的耐药性表达（所有β-内酰胺类抗生素耐药性的标志），所有菌株被分类为对甲氧西林均一耐药或异质性耐药（耐甲氧西林金黄色葡萄球菌-MRSA）以及对该抗生素敏感（甲氧西林敏感金黄色葡萄球菌-MSSA）。所有菌株根据对15种抗葡萄球菌药物的耐药模式、结晶紫试验结果、噬菌体模式以及通过PhenePlate（PhP）系统（BioSys inova）进行的生化指纹图谱结果进行分析。所有分析的MSSA对青霉素耐药，22%对四环素耐药，仅偶尔对其他抗菌药物耐药。它们对所有国际和额外裂解群的噬菌体敏感。异质性MRSA除对四环素外几乎对所有抗菌药物广泛敏感，并被第一、第三国际群的噬菌体和额外噬菌体裂解。所有这些菌株都产生青霉素酶。均一性MRSA具有多重耐药性，不能被第一和第二裂解群的噬菌体分型，能被第三和额外群的噬菌体弱分型。PhP系统将所有使用的菌株分为13种常见（两种占主导）和50种单菌株PhP型。各种表型分析方法的组合可能有助于医院的流行病学调查。
