Anderson A E, Hrachovy R A, Swann J W
Cain Foundation Laboratories, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.
Epilepsy Res. 1997 Mar;26(3):433-42. doi: 10.1016/s0920-1211(96)01010-8.
Studies were undertaken to compare tetanus toxin-induced electrographic and behavioral seizure activity in immature and adult rats. Multifocal epileptiform activity was induced by unilateral hippocampal injection of tetanus toxin in 10-day-old immature and in 90-120-day-old adult rats. Bilateral cortical surface electrodes and, in some animals, hippocampal depth electrodes were placed and serial video EEG was recorded for 2 h during the week following tetanus toxin injection. Tetanus toxin-induced interictal spike frequency was significantly higher and seizure duration was significantly longer in immature compared to adult rats. Immature rats tended to have more frequent seizures compared with adults, but the difference was not statistically significant. Ictal behaviors were different for the two groups with immature rats commonly demonstrating wild running seizures which were not seen in adult rats. These findings suggest that there are age-dependent differences in electrographic and behavioral seizure expression in the tetanus toxin seizure model and that the epileptic syndrome in immature rats is more severe.
开展了多项研究以比较破伤风毒素诱导的未成熟大鼠和成年大鼠的脑电图及行为性癫痫活动。通过向10日龄未成熟大鼠和90 - 120日龄成年大鼠单侧海马注射破伤风毒素来诱导多灶性癫痫样活动。放置双侧皮质表面电极，并在部分动物中放置海马深度电极，在注射破伤风毒素后的一周内连续记录2小时的视频脑电图。与成年大鼠相比，未成熟大鼠中破伤风毒素诱导的发作间期棘波频率显著更高，发作持续时间显著更长。与成年大鼠相比，未成熟大鼠的癫痫发作往往更频繁，但差异无统计学意义。两组的发作期行为有所不同，未成熟大鼠常见狂奔样发作，而成年大鼠未见此类发作。这些发现表明，在破伤风毒素癫痫模型中，脑电图和行为性癫痫表现存在年龄依赖性差异，且未成熟大鼠的癫痫综合征更为严重。
