Kanda Y, Akiyama H, Onozawa Y, Motegi T, Yamagata-Murayama S, Yamaguchi H
Division of Hematology and Pathology, Tokyo Metropolitan Komagome Hospital, Japan.
Kansenshogaku Zasshi. 1997 Mar;71(3):269-72. doi: 10.11150/kansenshogakuzasshi1970.71.269.
A patient in a blastic phase of chronic myelocytic leukemia developed multiple arterial emboli that originated from mitral valve vegetation. The diagnosis of infective endocarditis was not confirmed because blood cultures, serological assays and other examinations detected no pathogens. He died of intracranial hemorrhage after thrombolytic manipulation for embolization of the abdominal aorta and an autopsy was performed. Polymerase chain reaction analysis and Southern blot analysis of tissues from the mitral valve revealed Aspergillus species as the cause of the endocarditis, although none of the tissue specimens were culture-positive. These molecular analyses will be useful in the diagnosis of various types of Aspergillus infections.
一名慢性粒细胞白血病急变期患者出现多个动脉栓塞，栓子起源于二尖瓣赘生物。由于血培养、血清学检测及其他检查均未检测到病原体，感染性心内膜炎的诊断未得到证实。他在接受腹主动脉栓塞溶栓治疗后死于颅内出血，并进行了尸检。二尖瓣组织的聚合酶链反应分析和Southern印迹分析显示曲霉菌属是心内膜炎的病因，尽管所有组织标本培养均为阴性。这些分子分析将有助于诊断各种类型的曲霉菌感染。
