一种用玻璃绝缘微丝构建记录-注射微电极的简单方法。

A simple method for the construction of a recording-injection microelectrode with glass-insulated microwire.

作者信息

Tsai M L, Chai C Y, Yen C T

机构信息

Institute of Biomedical Sciences, Academica Sinica, Taipei, Taiwan, ROC.

出版信息

J Neurosci Methods. 1997 Mar;72(1):1-4. doi: 10.1016/s0165-0270(96)02151-6.

DOI:10.1016/s0165-0270(96)02151-6

PMID:9128161

Abstract

A rapid method for the production of a glass-insulated microwire electrode is described. A microwire was threaded into a glass capillary which was then pulled on a vertical pipette puller. A conical tip of the microwire was formed when the strongly heated glass capillary broke together with the wire in it. A tight seal of the glass-insulated microwire electrode between the glass and the metal was accomplished with silicone glue. The manufactured electrode performed consistently at different immersion depths, and yielded stable recordings of single units in the cerebral cortex and the medulla of rats. The strength and low impedance characteristics of the glass-insulated microwire electrode may make it useful for the recording of single units in deep brain structures. Furthermore, the electrode can be easily combined with another glass micropipette to form a dual recording-injection microelectrode unit.

摘要

本文描述了一种快速制备玻璃绝缘微丝电极的方法。将一根微丝穿入玻璃毛细管中，然后在垂直拉针仪上拉制。当强烈加热的玻璃毛细管与其中的微丝一起断裂时，微丝形成了一个锥形尖端。用硅胶胶水实现了玻璃绝缘微丝电极在玻璃和金属之间的紧密密封。所制造的电极在不同的浸入深度下表现一致，并能稳定记录大鼠大脑皮层和延髓中的单个神经元。玻璃绝缘微丝电极的强度和低阻抗特性可能使其适用于记录深部脑结构中的单个神经元。此外，该电极可以很容易地与另一个玻璃微吸管组合，形成一个双记录-注射微电极单元。