Saadati M, Gibbs H A, Parton R, Coote J G
Division of Infection and Immunity, University of Glasgow.
J Med Microbiol. 1997 Apr;46(4):276-84. doi: 10.1099/00222615-46-4-276.
Pasteurella haemolytica isolates from cattle and sheep, including representatives of different serotypes and untypable strains, were examined for leukotoxin (Lkt) production at the end of the log phase of growth in brain heart infusion broth. There were marked differences in leukotoxic activity in culture supernate samples, as measured by chemiluminescence-inhibition assays with bovine and ovine neutrophils, even between strains of the same serotype. There was also some variation in the amount and mol. wt of the Lkt protein produced by different strains, as judged by SDS-PAGE, immunoblotting and ELISA. Some strains produced normal amounts of Lkt protein which had only low leukotoxic activity. Most strains produced Lkt of 105 kDa whereas four strains produced a higher mol. wt form of c. 108 kDa, including two of the five serotype A2 strains examined. Thus, the P. haemolytica isolates showed considerable heterogeneity in terms of leukotoxin production, mol. wt and activity, even within a given serotype.
对从牛和羊中分离出的溶血巴斯德氏菌菌株进行了检测，这些菌株包括不同血清型的代表菌株以及无法分型的菌株，检测它们在脑心浸液肉汤中生长对数期结束时的白细胞毒素（Lkt）产生情况。通过用牛和羊的中性粒细胞进行化学发光抑制试验来测定培养上清液样本中的白细胞毒素活性，结果发现即使是同一血清型的菌株之间，其活性也存在显著差异。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）、免疫印迹和酶联免疫吸附测定（ELISA）判断，不同菌株产生的Lkt蛋白在量和分子量方面也存在一些差异。一些菌株产生的Lkt蛋白量正常，但白细胞毒素活性较低。大多数菌株产生的Lkt分子量为105 kDa，而有四个菌株产生了分子量约为108 kDa的较高分子量形式，其中包括所检测的五个血清型A2菌株中的两个。因此，即使在给定的血清型内，溶血巴斯德氏菌分离株在白细胞毒素产生、分子量和活性方面也表现出相当大的异质性。
