Sweeney S J, Silflow R M, Foreyt W J
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
J Wildl Dis. 1994 Oct;30(4):523-8. doi: 10.7589/0090-3558-30.4.523.
Twenty-eight isolates of Pasteurella haemolytica from domestic sheep (n = 14 isolates) and bighorn sheep (n = 14 isolates) were evaluated for leucotoxicity against peripheral blood neutrophils of bighorn sheep by adding bacterial culture supernatants to bighorn sheep neutrophils in vitro. Leukotoxic isolates of P. haemolytica, defined as causing > 50% neutrophil death as measured by release of lactate dehydrogenase into culture supernatants, were identified from eight of 14 domestic sheep isolates and from 0 of 14 bighorn sheep isolates. The in vitro assay of isolates of P. haemolytica may provide a valid predictive measure of strain virulence of P. haemolytica, and of potential pneumonic episodes in bighorn sheep populations.
通过在体外将细菌培养上清液添加到大角羊的外周血中性粒细胞中，对来自家羊（n = 14株）和大角羊（n = 14株）的28株溶血巴斯德菌进行了针对大角羊外周血中性粒细胞的白细胞毒性评估。溶血巴斯德菌的白细胞毒性菌株定义为通过培养上清液中乳酸脱氢酶的释放测定导致> 50%中性粒细胞死亡，在家羊的14株分离株中有8株被鉴定为白细胞毒性菌株，而在大角羊的14株分离株中未发现白细胞毒性菌株。溶血巴斯德菌分离株的体外测定可能为溶血巴斯德菌菌株毒力以及大角羊种群中潜在肺炎发作提供有效的预测指标。
