Bürgisser P, Spertini F, Weyrich-Suter C, Pagani J L, Meylan P R
Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
J Infect Dis. 1997 May;175(5):1202-5. doi: 10.1086/593568.
Fine-needle aspiration was used to collect lymph node cells (LNC) from 9 antiretroviral-naive patients entering a double-blind single- or combined-drug study of zidovudine, zalcitabine, and saquinavir. LNC were obtained twice before and 1 and 6 months after initiation of treatment. The effect of antiretroviral treatment on virus load ranged from no response to a dramatic decrease in plasma and LNC human immunodeficiency virus (HIV) RNA levels. The decrease in unspliced or spliced (or both) HIV RNAs in LNC was correlated with but consistently smaller than the decrease in plasma viremia. When present, the increase in blood CD4 T cells was, in general, moderate and transient. However, a striking rise in blood CD4 T cell count and in LNC CD4:CD8 ratio was observed in the 1 patient with the deepest sustained decrease in HIV RNA level in both plasma and lymph nodes.
采用细针穿刺法从9名初治抗逆转录病毒治疗患者中采集淋巴结细胞（LNC），这些患者进入了一项关于齐多夫定、扎西他滨和沙奎那韦的双盲单药或联合用药研究。在开始治疗前两次、治疗开始后1个月和6个月采集LNC。抗逆转录病毒治疗对病毒载量的影响范围从无反应到血浆和LNC中人类免疫缺陷病毒（HIV）RNA水平显著下降。LNC中未剪接或剪接（或两者）的HIV RNA的减少与血浆病毒血症的减少相关，但始终小于血浆病毒血症的减少。当出现时，血液中CD4 T细胞的增加通常是中度和短暂的。然而，在1例血浆和淋巴结中HIV RNA水平持续下降最深的患者中，观察到血液中CD4 T细胞计数和LNC中CD4:CD8比值显著升高。
