在四种鼠源抗痢疾志贺氏菌1型O-多糖抗体中，有三种抗体所采用的V基因与第四种抗体的V基因有很大差异。

Of four murine, anti-Shigella dysenteriae type 1 O-polysaccharide antibodies, three employ V-genes that differ extensively from those of the fourth.

作者信息

Miller C E, Karpas A, Schneerson R, Huppi K, Kováĉ P, Pozsgay V, Glaudemans C P

机构信息

Laboratory of Medicinal Chemistry, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Mol Immunol. 1996 Nov;33(16):1217-22. doi: 10.1016/s0161-5890(96)00105-8.

DOI:10.1016/s0161-5890(96)00105-8

PMID:9129157

Abstract

Three murine, monoclonal antibodies, IgM 5286 F2, IgM 5297 C1, and IgG 5338 H4 were generated against Shigella dysenteriae type 1 O-specific polysaccharide (O-SP)-conjugate. They are specific for the O-SP, which is a poly-[alpha-L-rhamnopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-al pha-D-galactopyranosyl-(1-->3)-2-deoxy-2-amino-N-acetyl-alpha-D-glucopyr anosyl]. The VH and VL genes of these antibodies were cloned and their sequences determined. They showed 93% homology, but were quite different to the primary sequence of IgM 3707 E9, of the same O-SP-specificity, previously reported. The fine-specificities of both IgG 5338 H4 and IgM 3707 E9 were for the same disaccharide moiety in the O-SP, while IgMs 5286 F2 and 5297 C1 showed fine-specificity for the entire repeating unit of the O-SP. Therefore, divergent sequences can confer upon antibodies similar-, or even identical-carbohydrate-epitope fine-specificity. In addition, close primary sequence-homology does not preclude differences in antibody fine-specificity.

摘要

针对痢疾志贺氏菌1型O特异性多糖（O-SP）-共轭物产生了三种鼠单克隆抗体，即IgM 5286 F2、IgM 5297 C1和IgG 5338 H4。它们对O-SP具有特异性，O-SP是一种聚-[α-L-鼠李吡喃糖基-(1→3)-α-L-鼠李吡喃糖基-(1→2)-α-D-吡喃半乳糖基-(1→3)-2-脱氧-2-氨基-N-乙酰-α-D-吡喃葡萄糖基]。克隆了这些抗体的重链可变区（VH）和轻链可变区（VL）基因并测定了其序列。它们显示出93%的同源性，但与先前报道的具有相同O-SP特异性的IgM 3707 E9的一级序列有很大不同。IgG 5338 H4和IgM 3707 E9对O-SP中的相同二糖部分具有精细特异性，而IgM 5286 F2和5297 C1对O-SP的整个重复单元具有精细特异性。因此，不同的序列可以赋予抗体相似甚至相同的碳水化合物表位精细特异性。此外，紧密的一级序列同源性并不排除抗体精细特异性的差异。