Jackson C J, Fox A J, Wareing D R, Sutcliffe E M, Jones D M
Public Health Laboratory, Withington Hospital, Manchester.
Epidemiol Infect. 1997 Apr;118(2):81-9. doi: 10.1017/s0950268896007388.
Genomic profiles were obtained for 76 strains of Campylobacter jejuni isolated from bacteraemic patients in England and Wales over the period 1981-94. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis using a random cloned DNA probe, and by ribotyping with a PCR-generated C. jejuni 16S ribosomal DNA probe. Phenotypic characterization was achieved by heat-stable (HS) and heat-labile (HL) serogrouping, and Preston phagetyping and biotyping. The blood isolates were genomically heterogenous, with 24 RFLP/16S profiles occurring within the 76 strains. Forty-four percent of isolates belonged to one of three RFLP/16S genotypes, reflecting the patterns seen in faecal isolates, except that genotypes usually associated with the HS 1 antigen were uncommon. The two most prevalent genotypes, characteristic of HS 2 and HS 4 strains, showed similarity by cluster analysis. Further evidence was seen of associations between phenotypic and genotypic characters within some HS serogroups. Chromosomal profiling by RFLP analysis does not indicate that particular genotypes have a predisposition to invade the bloodstream.
对1981年至1994年期间从英格兰和威尔士菌血症患者中分离出的76株空肠弯曲菌进行了基因组分析。使用随机克隆的DNA探针通过限制性片段长度多态性（RFLP）分析进行基因分型，并使用PCR产生的空肠弯曲菌16S核糖体DNA探针进行核糖体分型。通过热稳定（HS）和热不稳定（HL）血清分组、普雷斯顿噬菌体分型和生物分型进行表型鉴定。血液分离株在基因组上是异质的，76株菌株中有24种RFLP/16S图谱。44%的分离株属于三种RFLP/16S基因型之一，这反映了粪便分离株中观察到的模式，但通常与HS 1抗原相关的基因型并不常见。两种最常见的基因型，即HS 2和HS 4菌株的特征，通过聚类分析显示出相似性。在一些HS血清群中还发现了表型和基因型特征之间关联的进一步证据。通过RFLP分析进行的染色体分析并未表明特定基因型易侵入血流。
