通过唾液酸转移酶（siaD）聚合酶链反应酶联免疫吸附测定法对B群和C群脑膜炎球菌感染进行非培养诊断及血清群鉴定
Non-culture diagnosis and serogroup determination of meningococcal B and C infection by a sialyltransferase (siaD) PCR ELISA.
作者信息
Borrow R, Claus H, Guiver M, Smart L, Jones D M, Kaczmarski E B, Frosch M, Fox A J
机构信息
Manchester Public Health Laboratory, Withington Hospital, UK.
出版信息
Epidemiol Infect. 1997 Apr;118(2):111-7. doi: 10.1017/s0950268896007261.
Rapid, non-culture, serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. The impending availability of polysaccharide-protein conjugate vaccines for serogroup C disease requires maximal case ascertainment, with serogroup determination, at a time when the number of culture confirmed meningococcal infections is decreasing. A polymerase chain reaction assay (PCR), based on a restriction fragment length polymorphism (RFLP) in the meningococcal serogroup B and C sialytransferase (siaD) gene, was developed to combine the non-culture diagnosis of meningococcal infection from CSF, whole blood and serum with serogroup (B and C) identification. The PCR assay was adapted to an ELISA format incorporating hybridization with serogroup-specific B and C oligonucleotide probes. Specificity for CSFs was 100% and sensitivities were respectively 81, 63 and 30% for CSFs, whole blood and sera. The serogroup-specific PCR ELISA is a significant addition to currently available tests for non-culture diagnosis of meningococcal infection and outbreak investigation.
在可能进行疫苗接种的接触者管理中，快速、非培养的脑膜炎球菌感染血清群鉴定非常重要。对于C群疾病，多糖蛋白结合疫苗即将上市，这就需要在培养确诊的脑膜炎球菌感染病例数减少时，通过血清群鉴定尽可能多地确诊病例。基于脑膜炎球菌B群和C群唾液酸转移酶（siaD）基因的限制性片段长度多态性（RFLP）开发了一种聚合酶链反应检测法（PCR），将脑脊液、全血和血清中脑膜炎球菌感染的非培养诊断与血清群（B群和C群）鉴定相结合。该PCR检测法采用ELISA形式，结合了与血清群特异性B群和C群寡核苷酸探针的杂交。脑脊液的特异性为100%，脑脊液、全血和血清的敏感性分别为81%、63%和30%。血清群特异性PCR ELISA是目前用于脑膜炎球菌感染非培养诊断和疫情调查的现有检测方法的一项重要补充。
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