Meiyu F, Huosheng C, Cuihua C, Xiaodong T, Lianhua J, Yifei P, Weijun C, Huiyu G
Medical Research Institute, Yan-ling, Guangzhou, China.
Microbiol Immunol. 1997;41(3):209-13. doi: 10.1111/j.1348-0421.1997.tb01192.x.
Using a universal primer set designed to match the sequence of the NS1 gene of flaviviruses, the virus RNA of dengue (DEN), Japanese encephalitis (JEV), powassan and langat of Flaviviridae were successfully amplified by polymerase chain reaction (PCR) via cDNA; and with different internal primers, the serotypes of the dengue viruses were identified. Of the 78 clinically diagnosed dengue fever patients, 18 patients were positive for DEN 1, 48 patients for DEN 2 and 8 patients concurrently infected with DEN 4. Of the 52 patients admitted with Japanese encephalitis (JE), 45 were determined to be JEV infections. By nested PCR, we completed the identification of flaviviruses within 2 days. The results show that seven primers have a potential value for rapid clinical diagnosis of flavivirus infections.
使用设计用于匹配黄病毒NS1基因序列的通用引物组，通过聚合酶链反应（PCR）经cDNA成功扩增了黄病毒科登革热（DEN）、日本脑炎（JEV）、波瓦桑病毒和兰加特病毒的病毒RNA；并使用不同的内部引物鉴定了登革病毒的血清型。在78例临床诊断为登革热的患者中，18例DEN 1阳性，48例DEN 2阳性，8例同时感染DEN 4。在52例因日本脑炎（JE）入院的患者中，45例被确定为JEV感染。通过巢式PCR，我们在2天内完成了黄病毒的鉴定。结果表明，七种引物对黄病毒感染的快速临床诊断具有潜在价值。
