Gokulan K, Tripathi S P, Rao D N
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
Microbiol Immunol. 1997;41(3):215-20. doi: 10.1111/j.1348-0421.1997.tb01193.x.
The reactivity of antibodies with dimeric and monomeric peptide antigens was compared by ELISA. A panel of highly purified synthetic peptides of HIV-1 representing defined regions, 598-609 and 524-533 (fusion domain) of gp41 and 306-320 of gp120, were used as antigens in the ELISA. These peptides were selected and synthesized taking into account the level of sequence conservation of various strains and hydrophilicity. The analysis included sera from 52 HIV-1 infected individuals and 53 HIV-1 negative controls. Both peptides from gp41 were found to be particularly immunoreactive with sera from HIV-1 infected individuals. The frequency of reactivity to the selected peptide from gp120 (V3 loop) in infected individuals was 82%. An interesting observation was that the dimeric peptide antigens had a detection rate more than 4-fold higher than the monomeric antigens. We found that lower levels of antibodies could be detected with dimeric antigens. The peptides reacted with few sera other than HIV-1 positive sera. These results implicate the potential dimeric peptide antigens to be utilized in the serodiagnosis of HIV-1 infection.
通过酶联免疫吸附测定（ELISA）比较了抗体与二聚体和单体肽抗原的反应性。一组高度纯化的代表HIV-1特定区域的合成肽，即gp41的598-609和524-533（融合结构域）以及gp120的306-320，被用作ELISA中的抗原。这些肽的选择和合成考虑了各种毒株的序列保守水平和亲水性。分析包括来自52名HIV-1感染个体和53名HIV-1阴性对照的血清。发现来自gp41的两种肽与HIV-1感染个体的血清具有特别强的免疫反应性。感染个体中对来自gp120（V3环）的选定肽的反应频率为82%。一个有趣的观察结果是，二聚体肽抗原的检测率比单体抗原高4倍以上。我们发现使用二聚体抗原可以检测到较低水平的抗体。这些肽除了与HIV-1阳性血清外，与其他血清反应很少。这些结果表明二聚体肽抗原在HIV-1感染的血清学诊断中具有潜在的应用价值。
