Ido E, Okada M, Enose Y, Kuwata T, Igarashi T, Sata T, Terao K, Chen J, Hayami M
Laboratory of Pathogenic Virus, University, Japan.
Microbiol Immunol. 1997;41(3):277-80. doi: 10.1111/j.1348-0421.1997.tb01201.x.
We previously reported that an HIV-1/SIVmac chimeric virus (designated as NM-3rN) having HIV-1 env efficiently infected macaque monkeys by intravenous inoculation. In this study, this chimeric virus was atraumatically inoculated into the vaginal cavity of two rhesus and one cynomolgus monkeys. Although antibody response and detection of proviral genome by PCR were observed in both rhesus monkeys, virus recovery was only once from PBMC in one of them. In the cynomolgus monkey, no virus was recovered and proviral DNA detection was rare. Thus, vaginal inoculation with NM-3rN resulted in poor systemic infection, implying the presence of selective pressure while passing through mucosal membranes.
我们之前报道过，一种具有HIV-1包膜的HIV-1/SIVmac嵌合病毒（命名为NM-3rN）通过静脉接种能有效感染猕猴。在本研究中，将这种嵌合病毒经无创方式接种到两只恒河猴和一只食蟹猴的阴道内。虽然在两只恒河猴中均观察到了抗体反应以及通过PCR检测到前病毒基因组，但其中只有一只恒河猴的外周血单核细胞（PBMC）中仅一次检测到病毒。在食蟹猴中，未检测到病毒，且前病毒DNA检测结果罕见。因此，用NM-3rN进行阴道接种导致全身感染情况不佳，这意味着在通过黏膜时存在选择性压力。
