屋尘螨过敏原多态性的功能效应

Functional effects of polymorphisms of house dust mite allergens.

作者信息

Thomas W R, Smith W, Hales B J, Carter M D

机构信息

Division of Molecular Biology, TVW Telethon Institute for Child Health Research, West Perth, Australia.

出版信息

Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):96-8. doi: 10.1159/000237516.

DOI:10.1159/000237516

PMID:9130492

Abstract

BACKGROUND

Sequence polymorphisms have been found in cDNA clones of commercial house dust mites. Here we report on the polymorphisms of Der p 1 found in environmental mites and the effect of the polymorphisms on T cell proliferation.

METHODS

Sequences were obtained from DNA amplified from single mites by PCR. Synthetic peptides were used to stimulate peripheral blood cells.

RESULTS

The polymorphism was limited to a few residues, but regional differences were detected in mites in Wagga. T cells were specific for the polymorphisms, so that in 16 of 17 responses, stimulation was achieved with one form of the peptide. This included peptides with Ala and Val at position 124 in the immunodominant 110-133 region. Peptide 45-63 also stimulated frequently, but only with Tyr at position 50 and not His, a residue only found in commercial mites.

CONCLUSIONS

Polymorphisms of Der p 1 are found in the environment and have a profound effect on polyclonal T cell stimulation to frequently recognised epitopes.

摘要

背景

在商业用尘螨的cDNA克隆中发现了序列多态性。在此，我们报告环境螨中Der p 1的多态性及其对T细胞增殖的影响。

方法

通过PCR从单个螨扩增的DNA中获得序列。合成肽用于刺激外周血细胞。

结果

多态性仅限于少数几个残基，但在瓦加的螨中检测到区域差异。T细胞对多态性具有特异性，因此在17次反应中的16次中，一种形式的肽可实现刺激。这包括免疫显性110 - 133区域中第124位为丙氨酸和缬氨酸的肽。肽45 - 63也经常刺激，但仅在第50位为酪氨酸时刺激，而非组氨酸，组氨酸仅在商业螨中发现。