Welker P, Grabbe J, Zuberbier T, Henz B M
Department of Dermatology, Virchow Clinics, Humboldt University, Berlin, Germany.
Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):284-6. doi: 10.1159/000237574.
Fibroblast-derived growth factors like SCF are able to upregulate the expression of mast cell characteristics in human multilineage hematopoietic progenitor cells. Other factors, like GM-CSF, have been reported to inhibit this process, probably by the competitive recruitment of cells not belonging to the mast cell lineage. In this study, we investigated the influence of GM-CSF on immature mast cells of the HMC-1 cell line which already show low-level expression of mast cell tryptase, histamine and Fc epsilonRI alpha. Culture of HMC-1 cells with mast-cell-conditioning medium, containing fibroblast supernatants, upregulated tryptase activity, histamine contents and expression of Fc epsilonRI alpha. However, addition of GM-CSF (10 ng/ml) markedly downregulated these mast cell markers, without affecting proliferation and viability of cells. Thus, GM-CSF may provide an inhibitory signal during mast cell differentiation and probably even downregulates mast cell characteristics in more differentiated cells.
像干细胞因子（SCF）这样的成纤维细胞衍生生长因子能够上调人类多谱系造血祖细胞中肥大细胞特征的表达。据报道，其他因子，如粒细胞-巨噬细胞集落刺激因子（GM-CSF），可能通过竞争性募集不属于肥大细胞谱系的细胞来抑制这一过程。在本研究中，我们调查了GM-CSF对HMC-1细胞系未成熟肥大细胞的影响，该细胞系已表现出肥大细胞类胰蛋白酶、组胺和FcεRIα的低水平表达。用含有成纤维细胞上清液的肥大细胞条件培养基培养HMC-1细胞，可上调类胰蛋白酶活性、组胺含量和FcεRIα的表达。然而，添加GM-CSF（10 ng/ml）可显著下调这些肥大细胞标志物，而不影响细胞的增殖和活力。因此，GM-CSF可能在肥大细胞分化过程中提供抑制信号，甚至可能下调更分化细胞中的肥大细胞特征。
