从人肥大细胞增多症细胞系获得的条件培养基可诱导人祖细胞来源的肥大细胞表达糜蛋白酶，但不表达类胰蛋白酶。
Conditioned media obtained from a human mastocytosis cell strain induce mast cells expressing chymase but not tryptase from human progenitors.
作者信息
Li L, Krilis S A
机构信息
School of Medicine, University of New South Wales and Department of Immunology, Allergy and Infectious Disease, St. George Hospital, Kogarah, Australia.
出版信息
Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):289-90. doi: 10.1159/000237576.
Human mast cells (MC) were derived from umbilical cord blood and bone marrow progenitors cultured in the presence of a conditioned medium from a human mastocytosis cell strain and recombinant human kit ligand (rhKL). KL induced MC of predominantly two immunophenotypes, MC(T) and MC(TC). In contrast, the conditioned medium induced MC subtypes MC(TC) and a third subtype, MC(C), positive for chymase but negative for tryptase. This study clearly demonstrates that a third type of MC, MC(C), can be induced in vitro from normal human progenitors.
人肥大细胞（MC）来源于脐血和骨髓祖细胞，这些祖细胞在人肥大细胞增多症细胞系的条件培养基和重组人干细胞因子配体（rhKL）存在的情况下进行培养。干细胞因子诱导产生的肥大细胞主要有两种免疫表型，即MC(T)和MC(TC)。相比之下，条件培养基诱导产生的肥大细胞亚型为MC(TC)和第三种亚型MC(C)，MC(C)对糜蛋白酶呈阳性反应，对组织蛋白酶呈阴性反应。这项研究清楚地表明，第三种类型的肥大细胞，即MC(C)，可以在体外由正常人祖细胞诱导产生。
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