Bonafonte M T, Priest J W, Garmon D, Arrowood M J, Mead J R
Emory University, Atlanta, GA 30033, USA.
Biochim Biophys Acta. 1997 Apr 10;1351(3):256-60. doi: 10.1016/s0167-4781(97)00013-4.
A gene encoding for Cryptosporidium parvum (C. parvum) elongation factor 1alpha (EF-1alpha) was isolated and sequenced from a cDNA expression library. The recombinant protein cross-reacted with a monoclonal antibody that was raised to a sporozoite cell surface antigen. The gene encoded a 435 amino acid protein with a predicted molecular weight of 48.1 kDa. The predicted C. parvum EF-1alpha protein sequence showed extensive homology with the EF-1alpha proteins of other eukaryotic organisms and included three conserved sequence motifs implicated in GTP binding.
