Lobina C, Agabio R, Diaz G, Fa M, Fadda F, Gessa G L, Reali R, Colombo G
Bernard B. Brodie, Department of Neuroscience, University of Cagliari, Italy.
Alcohol Alcohol. 1997 Jan-Feb;32(1):19-22. doi: 10.1093/oxfordjournals.alcalc.a008229.
The present study was designed to evaluate ethanol drinking behaviour in Sardinian alcohol-preferring (sP) and Sardinian alcohol-non-preferring (sNP) rats in the presence of different ethanol concentrations. Ethanol intake was tested under the two-bottle, free-choice regimen and continuous access schedule. Ethanol-naive sP and sNP rats were initially given ethanol solution at the standard, constant concentration of 10% (v/v) for 8 consecutive days (Phase 1). As expected, daily ethanol intake in sP rats rose from 4 to approximately 6 g/kg; in contrast sNP rats consumed < 10 g/kg/day ethanol. Subsequently, an ascending series of ethanol concentrations, ranging from 3 to 60% (v/v), was presented to sP and sNP rats over a 28-day period (Phase 2). At concentrations varying from 7 to 30%, sP rats consumed constant amounts of absolute ethanol per kg of body weight (approximately 6.0 g/kg/day). Daily ethanol intake in sNP rats remained constantly lower than 1.0 g/kg, irrespective of the ethanol concentration. Data from Phase 2 demonstrate the ability of sP rats to precisely adjust daily ethanol intake and support the hypothesis that voluntary ethanol drinking in sP rats is sustained by specific pharmacological effects of ethanol.
本研究旨在评估在不同乙醇浓度条件下，撒丁岛嗜酒（sP）大鼠和撒丁岛不嗜酒（sNP）大鼠的乙醇饮用行为。乙醇摄入量在双瓶自由选择方案和持续获取时间表下进行测试。未接触过乙醇的sP和sNP大鼠最初连续8天给予浓度为10%（v/v）的标准恒定乙醇溶液（第1阶段）。正如预期的那样，sP大鼠的每日乙醇摄入量从4克/千克增加到约6克/千克；相比之下，sNP大鼠每天摄入的乙醇<10克/千克。随后，在28天的时间里，向sP和sNP大鼠提供一系列浓度逐渐升高的乙醇，范围从3%到60%（v/v）（第2阶段）。在7%到30%的不同浓度下，sP大鼠每千克体重消耗的纯乙醇量恒定（约6.0克/千克/天）。无论乙醇浓度如何，sNP大鼠的每日乙醇摄入量始终低于1.0克/千克。第2阶段的数据证明了sP大鼠精确调整每日乙醇摄入量的能力，并支持了sP大鼠自愿饮用乙醇是由乙醇的特定药理作用维持的这一假设。
