Okada M, Ito Y, Inano K, Miida T, Matsuto T
Department of Laboratory Medicine, Niigata University School of Medicine, Japan.
Ann Clin Biochem. 1997 Mar;34 ( Pt 2):173-8. doi: 10.1177/000456329703400208.
It is well recognized that oxidative modification of low-density lipoprotein (LDL) accelerates atherogenesis of the arterial vascular wall. In this study, we examined the relationship between various methods of measuring the extent of oxidation, namely lipid peroxidation products, surface charge, and spectrophotometric patterns. LDL was isolated from fresh human normal plasma by centrifugation and was oxidized by incubating with copper ions. Apolipoprotein B was isolated from the LDL solution. We also prepared artificial lipid particles composed of cholesterol linolenate, triolein, and phosphatidylcholine. Our results suggest that lipid peroxidation begins drastically in 30-60 min, while the abolition of the positive charge on apoliproproteins is accelerated after 60 min. We found a characteristic change in the spectrophotometric pattern during the process and conclude that the spectrophotometric absorption ration at 232 and 203 nm is a useful measure of in vitro oxidation of LDL.
众所周知，低密度脂蛋白（LDL）的氧化修饰会加速动脉血管壁的动脉粥样硬化形成。在本研究中，我们检测了各种测量氧化程度方法之间的关系，即脂质过氧化产物、表面电荷和分光光度模式。通过离心从新鲜人正常血浆中分离出LDL，并通过与铜离子孵育使其氧化。从LDL溶液中分离出载脂蛋白B。我们还制备了由胆固醇亚麻酸酯、三油酸甘油酯和磷脂酰胆碱组成的人工脂质颗粒。我们的结果表明，脂质过氧化在30 - 60分钟内急剧开始，而载脂蛋白上正电荷的消除在60分钟后加速。我们在该过程中发现分光光度模式有特征性变化，并得出结论，232和203nm处的分光光度吸收比是体外LDL氧化的有用指标。
