Valerón P F, Chirino R, Vega V, Falcón O, Rivero J F, Torres S, León L, Fernández L, Pestano J, Díaz-Chico B, Díaz-Chico J C
Department of Cellular and Molecular Endocrinology, University of Las Palmas de Gran Canaria, Canary Islands, Spain.
Int J Cancer. 1997 Apr 22;74(2):175-9. doi: 10.1002/(sici)1097-0215(19970422)74:2<175::aid-ijc6>3.0.co;2-w.
The total cellular p185(HER-2/neu) protein (p185) content was measured by ELISA in 346 invasive primary breast cancers, and the results were compared with those of estrogen (ER) and progesterone (PR) receptors, pS2 and Cathepsin D (Cat D) content. At a cut-off level of 260 fmol/mg protein, 53 of the 346 tumors (15%) were p185-positive. A significant positive correlation was observed between p185 levels and those of Cat D, and a weaker, though significant, positive correlation with ER, and pS2 levels, but not with those of PR. However, when only the 293 p185-negative tumors were considered, the correlation between p185 and ER improved substantially, and statistical significance was reached for PR. p185-positive tumors exhibited lower ER and PR content and higher Cat D content than p185-negative tumors. The pS2 content, in contrast, did not undergo significant variation. Tumors considered to be p185-positive were significantly more frequently positive for Cat D at the cut-off of 45 pmol/mg protein, and were more frequently negative for ER and/or PR, but only significant at the cut-off of 15 fmol/mg or higher for both steroid receptors. Finally, p185 status was not associated with menopausal status, tumor size, axillary-lymph-node invasiveness or distant metastases. These results suggest that 260 fmol/mg protein as the cut-off for p185 allows the identification of a tumoral sub-population with a more aggresive phenotype.
采用酶联免疫吸附测定法（ELISA）检测了346例浸润性原发性乳腺癌细胞中总p185（HER-2/neu）蛋白（p185）含量，并将结果与雌激素（ER）、孕激素（PR）受体、pS2和组织蛋白酶D（组织蛋白酶D）含量进行比较。在蛋白含量截断值为260 fmol/mg时，346例肿瘤中有53例（15%）p185呈阳性。观察到p185水平与组织蛋白酶D水平之间存在显著正相关，与ER和pS2水平呈较弱但显著的正相关，与PR水平无相关性。然而，仅考虑293例p185阴性肿瘤时，p185与ER之间的相关性显著改善，PR也具有统计学意义。p185阳性肿瘤的ER和PR含量低于p185阴性肿瘤，组织蛋白酶D含量高于p185阴性肿瘤。相比之下，pS2含量没有显著变化。在蛋白含量截断值为45 pmol/mg时，被认为p185阳性的肿瘤组织蛋白酶D阳性的频率显著更高，ER和/或PR阴性的频率也更高，但仅在两种甾体受体截断值为15 fmol/mg或更高时具有统计学意义。最后，p185状态与绝经状态、肿瘤大小、腋窝淋巴结浸润或远处转移无关。这些结果表明，以260 fmol/mg蛋白作为p185的截断值，可以识别出具有更具侵袭性表型的肿瘤亚群。
