Inagaki M, Inagaki N, Takahashi T, Takai Y
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya.
J Biochem. 1997 Mar;121(3):407-14. doi: 10.1093/oxfordjournals.jbchem.a021603.
Site-specific phosphorylation of intermediate filament (IF) proteins on serine and threonine residues leads to dynamic alterations in filament structure. Site- and phosphorylation state-specific antibodies for IF proteins can visualize spatial and temporal distribution of site-specific IF protein phosphorylations in the cell. These antibodies are also useful to identity IF kinases involved in cellular events, including cell signaling and cell cycle.
中间丝（IF）蛋白在丝氨酸和苏氨酸残基上的位点特异性磷酸化导致丝结构的动态变化。针对IF蛋白的位点和磷酸化状态特异性抗体可以可视化细胞中位点特异性IF蛋白磷酸化的时空分布。这些抗体对于鉴定参与细胞事件（包括细胞信号传导和细胞周期）的IF激酶也很有用。
