培养的完整肝细胞将[14C]葡糖胺掺入二磷酸多萜醇N-乙酰[14C]葡糖胺的过程。

The incorporation of [14C]glucosamine into dolichol diphosphate N-acetyl[14C] glucosamine by unbroken liver cells in culture.

作者信息

Cooper J R, Hemming F W

出版信息

Eur J Biochem. 1977 Aug 15;78(1):89-94. doi: 10.1111/j.1432-1033.1977.tb11716.x.

DOI:10.1111/j.1432-1033.1977.tb11716.x

Abstract

Incubation of whole Chang liver cells with D-[1-14C]glucosamine results in incorporation of radioactivity into both proteins and lipids. A minor (approximately 3%) amount of the labelled lipid has the chromatographic, solubility and chemical properties of dolichol diphosphate N-acetylglucosamine. A similar compound is formed when membrane preparations of the cells are incubated with UDP-N-acetyl[14C]glucosamine. The same membrane fractions catalyse the transfer of [14C]-mannose from GDP-[14C]mannose to dolichol phosphate.

摘要

用D-[1-¹⁴C]葡糖胺孵育整个张氏肝细胞，会导致放射性掺入蛋白质和脂质中。少量（约3%）标记脂质具有二磷酸多萜醇N-乙酰葡糖胺的色谱、溶解性和化学性质。当细胞的膜制剂与UDP-N-乙酰[¹⁴C]葡糖胺一起孵育时，会形成类似的化合物。相同的膜组分催化[¹⁴C]甘露糖从GDP-[¹⁴C]甘露糖转移至磷酸多萜醇。

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