通过使用各种哺乳动物物种的红细胞膜对糖鞘脂进行定量测定。
Quantitative determination of glycosphingolipids illustrated by using erythrocyte membranes of various mammalian species.
作者信息
Oulevey J, Bodden E, Thiele O W
出版信息
Eur J Biochem. 1977 Sep 15;79(1):265-7. doi: 10.1111/j.1432-1033.1977.tb11805.x.
Abstract
A procedure for the quantative determination of glycosphingolipids is described, involving extraction of total lipids, fractionation on a column, acid cleavage and photometry of a complex formed between sphingosine and methyl orange. The level of glycosphingolipid sphigosine of erythrocyte membranes of a variety of mammals ranges from 59.1 (rat) to 410.1 (pig) nmol/ml packed cells.
摘要
描述了一种定量测定糖鞘脂的方法，包括总脂质提取、柱层析分离、酸裂解以及对鞘氨醇与甲基橙形成的复合物进行光度测定。多种哺乳动物红细胞膜中糖鞘脂鞘氨醇的水平范围为每毫升压实细胞59.1（大鼠）至410.1（猪）纳摩尔。
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