Okey A B, Giannone J V, Smart W, Wong J M, Manchester D K, Parker N B, Feeley M M, Grant D L, Gilman A
Department of Pharmacology, University of Toronto, Ontario, Canada.
Chemosphere. 1997 Mar-Apr;34(5-7):1535-47. doi: 10.1016/s0045-6535(97)00449-9.
Binding of [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin to AH receptor was characterized in cytosol from human placentas in which the pregnancy outcome was normal compared with pregnancies in which there was some adverse outcome (premature birth; intrauterine growth retardation; structural abnormality). No significant difference was detected between normal and adverse outcomes in the concentration of AH receptor sites (Bmax) nor in the affinity with which [3H]TCDD bound to the receptor (Kd). Aryl hydrocarbon hydroxylase activity, a CYP1A1 enzyme regulated by the AH receptor, was elevated in placental microsomes from smokers; this elevation was associated with intrauterine growth retardation.
