Shochina M, Loesch A, Rubino A, Miah S, Macdonald G, Burnstock G
Rehabilitation Department, Hadassah University Hospital, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Cell Tissue Res. 1997 Jun;288(3):509-16. doi: 10.1007/s004410050836.
The ultrastructural localization of immunoreactivity to nitric oxide synthase (type-III and type-II) and endothelin-1 was examined by using pre-embedding peroxidase-antiperoxidase techniques in the coronary and cerebral basilar arteries in renal hypertensive rats. Renal hypertension was produced by excision of the right kidney and clipping of the left renal artery. Controls were normotensive sham-operated rats (right surgical nephrectomy; a clip inserted near the left renal artery). Both in controls and hypertensive rats, immunoreactivities for nitric oxide synthase-III and endothelin-1 were localized within subpopulations of endothelial cells. In addition, signs of translocation of nitric oxide synthase-III were noted from the cytoplasm to the Golgi complex in endothelial cells of the basilar artery of hypertensive animals. Neither controls nor hypertensive rats showed immunoreactivity for nitric oxide synthase-II. Preparations of the right coronary artery from hypertensive rats displayed fewer endothelial cells positive to nitric oxide synthase-III than in controls, although there were no significant changes in the distribution of endothelin-1-positive endothelial cells in the coronary artery of hypertensive rats. In contrast, the basilar artery from hypertensive rats displayed no changes in the percentage of endothelial cells immuno-positive either for nitric oxide synthase-III or for endothelin-1. In consequence, the ratio of nitric oxide synthase-III:endothelin-1 was reduced in the coronary but not in the basilar artery. Therefore, the nitric oxide/endothelin-1 system appears to play different roles in the coronary and cerebral circulations during renal hypertension.
采用包埋前过氧化物酶 - 抗过氧化物酶技术，对肾性高血压大鼠冠状动脉和脑基底动脉中一氧化氮合酶（Ⅲ型和Ⅱ型）及内皮素 - 1免疫反应性的超微结构定位进行了研究。通过切除右肾并夹闭左肾动脉制备肾性高血压模型。对照组为血压正常的假手术大鼠（右肾切除术；在左肾动脉附近插入夹子）。在对照组和高血压大鼠中，一氧化氮合酶Ⅲ型和内皮素 - 1的免疫反应性均定位于内皮细胞亚群内。此外，在高血压动物基底动脉的内皮细胞中，观察到一氧化氮合酶Ⅲ型从细胞质向高尔基体复合体的易位迹象。对照组和高血压大鼠均未显示一氧化氮合酶Ⅱ型的免疫反应性。高血压大鼠右冠状动脉的标本中，一氧化氮合酶Ⅲ型阳性的内皮细胞比对照组少，尽管高血压大鼠冠状动脉中内皮素 - 1阳性内皮细胞的分布没有显著变化。相比之下，高血压大鼠的基底动脉中，一氧化氮合酶Ⅲ型或内皮素 - 1免疫阳性的内皮细胞百分比没有变化。因此，一氧化氮合酶Ⅲ型与内皮素 - 1的比例在冠状动脉中降低，但在基底动脉中未降低。所以，在肾性高血压期间，一氧化氮/内皮素 - 1系统似乎在冠状动脉和脑循环中发挥不同作用。
