Honer W G, Falkai P, Young C, Wang T, Xie J, Bonner J, Hu L, Boulianne G L, Luo Z, Trimble W S
Department of Psychiatry, University of British Columbia, Vancouver, Canada.
Neuroscience. 1997 May;78(1):99-110. doi: 10.1016/s0306-4522(96)00489-7.
The neuronal organization and patterns of afferent innervation are abnormal in the cingulate cortex in schizophrenia, and associated changes in synaptic terminals could be present. A panel of monoclonal antibodies was defined with biochemical and fusion protein studies as detecting syntaxin (antibody SP6), synaptophysin (antibody SP4) and synaptosomal-associated protein-25 (antibody SP12). These antibodies and a polyclonal antibody reactive with neural cell adhesion molecule were used to investigate the cingulate cortex in schizophrenia. Immunocytochemistry indicated that syntaxin immunoreactivity had a considerably wider distribution than synaptophysin. Overall, multivariate analysis indicated increased synaptic terminal protein immunoreactivity in schizophrenia compared to controls (P=0.004). Controlled for age and post mortem interval, syntaxin immunoreactivity was significantly elevated in schizophrenia (P=0.004), and neural cell adhesion molecule immunoreactivity was also elevated (P=0.05). The neural cell adhesion molecule to synaptophysin ratio was increased (P=0.005), possibly indicating the presence of less mature synapses in schizophrenia. Elevated syntaxin immunoreactivity is consistent with increased glutamatergic afferents to the cingulate cortex in schizophrenia, and combined with the neural cell adhesion molecule to synaptophysin ratio results suggests that synaptic function in this region in schizophrenia may be abnormal.
精神分裂症患者扣带回皮质的神经元组织和传入神经支配模式异常，可能存在突触终末的相关变化。通过生化和融合蛋白研究确定了一组单克隆抗体，可检测 syntaxin（抗体 SP6）、突触素（抗体 SP4）和突触体相关蛋白 25（抗体 SP12）。使用这些抗体以及一种与神经细胞黏附分子反应的多克隆抗体来研究精神分裂症患者的扣带回皮质。免疫细胞化学表明，syntaxin 的免疫反应性分布比突触素广泛得多。总体而言，多变量分析表明，与对照组相比，精神分裂症患者突触终末蛋白免疫反应性增加（P = 0.004）。在控制年龄和死后间隔后，精神分裂症患者的 syntaxin 免疫反应性显著升高（P = 0.004），神经细胞黏附分子免疫反应性也升高（P = 0.05）。神经细胞黏附分子与突触素的比率增加（P = 0.005），这可能表明精神分裂症患者存在不太成熟的突触。Syntaxin 免疫反应性升高与精神分裂症患者扣带回皮质谷氨酸能传入增加一致，结合神经细胞黏附分子与突触素比率的结果表明，精神分裂症患者该区域的突触功能可能异常。
