Amlacher E, Bierwolf D
Exp Pathol (Jena). 1977;13(2-3):88-100. doi: 10.1016/s0014-4908(77)80035-5.
In skin tumors induced in Syrian hamsters by Papova viruses or produced chemically by dripping of methylnitroso urea (MNU) the pattern of DNA synthesis was studied in vitro and in vivo by autoradiography. The greater part of DNA synthesis in the Papova tumor of the hamster is of cellular origin. Only the cells localized adjacent to keratinizing regions of the tumors may be considered as virus-infected with progressive maultiplication of viruses. This also applies to all nuclei with cellular DNA synthesis only in the marginal chromatin. Moreover viral DNA synthesis is supposed in the cytoplasm, too. In methylnitroso urea-induced squamous cell carcinoma labeled cells were likewise found adjacent to keratinizing tumor regions and the pattern of DNA synthesis is generally not limited to the "stratum basale". With increasing malignancy the pattern of DNA synthesis is changing also in chemically induced tumors and is no longer limited to the stratum basale where it still can be demonstrated in the papilloma.
在叙利亚仓鼠中由乳头多瘤空泡病毒诱导产生或通过滴注甲基亚硝基脲（MNU）化学诱导产生的皮肤肿瘤中，通过放射自显影术在体外和体内研究了DNA合成模式。仓鼠乳头多瘤空泡病毒肿瘤中DNA合成的大部分来源于细胞。只有位于肿瘤角质化区域附近的细胞可被视为病毒感染且病毒有进行性增殖。这也适用于仅在边缘染色质中有细胞DNA合成的所有细胞核。此外，推测细胞质中也有病毒DNA合成。在甲基亚硝基脲诱导的鳞状细胞癌中，同样在肿瘤角质化区域附近发现了标记细胞，并且DNA合成模式通常不限于“基底层”。随着恶性程度的增加，化学诱导肿瘤中的DNA合成模式也在发生变化，不再局限于在乳头状瘤中仍可显示的基底层。
