Matsuo R, Herndon D N, Kobayashi M, Pollard R B, Suzuki F
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77555, USA.
J Trauma. 1997 Apr;42(4):635-40. doi: 10.1097/00005373-199704000-00009.
We have described previously that CD8+ CD11b+ TCR gamma/delta+ type 2 T cells (BA-type 2 T cells) and suppressor macrophages (Sup-Mł) are generated in spleens of mice 3 to 5 days (Sup-Mł) and 4 to 9 days (BA-type 2 T cells) after thermal injury. In the present study, an additional suppressor T cell, characterized as CD3+ CD4- CD8- TCR alpha/beta+ T cells (double negative suppressor T cells, DN Sup-T cells), was demonstrated in mice 1 day after thermal injury. DN Sup-T cells inhibited the proliferation of lymphocytes stimulated with allogeneic cells or a lectin in a mixed lymphocyte reaction, and produced both type 1 and type 2 cytokines (interferon-gamma, interleukin-2, interleukin-4, and interleukin-10) when they were stimulated in vitro with anti-CD3 monoclonal antibody. These results suggest that DN Sup-T cells express phenotypic properties similar to natural suppressor cells and cytokine-producing profiles different from type 1 (Th1 cells and CTLs) and type 2 T cells (Th2 cells and CD8+ type 2 T cells). DN Sup-T cells may play a role on the burn-associated immunosuppression appearing in the acute phase of thermally injured individuals.
我们之前已经描述过，热损伤后3至5天（抑制性巨噬细胞）和4至9天（BA-2型T细胞）在小鼠脾脏中产生CD8 + CD11b + TCRγ/δ+ 2型T细胞（BA-2型T细胞）和抑制性巨噬细胞（Sup-Mł）。在本研究中，在热损伤后1天的小鼠中发现了另一种抑制性T细胞，其特征为CD3 + CD4- CD8- TCRα/β+ T细胞（双阴性抑制性T细胞，DN Sup-T细胞）。DN Sup-T细胞在混合淋巴细胞反应中抑制同种异体细胞或凝集素刺激的淋巴细胞增殖，并且当它们在体外被抗CD3单克隆抗体刺激时会产生1型和2型细胞因子（干扰素-γ、白细胞介素-2、白细胞介素-4和白细胞介素-10）。这些结果表明，DN Sup-T细胞表达的表型特性类似于天然抑制细胞，并且细胞因子产生谱不同于1型（Th1细胞和CTL）和2型T细胞（Th2细胞和CD8 + 2型T细胞）。DN Sup-T细胞可能在热损伤个体急性期出现的烧伤相关免疫抑制中发挥作用。
