Alaibac M, Congedo M, Barbarossa G, Bottiglieri A, Fillippi E D, Marzullo F, Quarta G, Schittulli F
Laboratory of Experimental Oncology, National Oncology Institute, Bari, Italy.
Anticancer Res. 1997 Mar-Apr;17(2A):1205-7.
Kaposi's sarcoma (KS) is a multifocal neoplasm of unknown origin. All forms of KS are composed of spindle-shaped cells with elongated nuclei and sheets of endothelial-like cells. The proliferation of spindle cells is accompanied by the presence of an inflammatory infiltrate composed predominantly of T-cells. It has been suggested that this infiltrate might consist of a virally stimulated clonal population of T-lymphocytes which can produce growth factors initiating and substaining the proliferation of spindle-shaped cells. In this study we analyzed for clonal T-cell receptor gama gene rearrangements the T-cell populations present in the cutaneous infiltrate of seven cases of classical Kaposi's sarcoma using a polymerase chain reaction-based approach. Our data demonstrate the lack of a significant clonal population of T-cells in the cutaneous infiltrates of KS. This finding is indicative of a reactive polyclonal response of T-cells to the spindle-shaped cells and supports the contention that spindle-shaped cells are pathogenetically the central cell type in the disease. Our data also indicate that the anti-KS T-cell response, being polyclonal in nature, does not result from clonal expansion of T-cells targeting tumor-associated antigenic peptides.
卡波西肉瘤（KS）是一种起源不明的多灶性肿瘤。所有类型的KS均由具有细长细胞核的梭形细胞和成片的内皮样细胞组成。梭形细胞的增殖伴随着主要由T细胞组成的炎性浸润。有人提出，这种浸润可能由病毒刺激的T淋巴细胞克隆群体组成，这些T淋巴细胞可产生启动和维持梭形细胞增殖的生长因子。在本研究中，我们使用基于聚合酶链反应的方法，分析了7例经典卡波西肉瘤皮肤浸润中存在的T细胞群体的克隆性T细胞受体γ基因重排。我们的数据表明，KS皮肤浸润中缺乏显著的T细胞克隆群体。这一发现表明T细胞对梭形细胞的反应是反应性多克隆反应，并支持梭形细胞在发病机制上是该疾病核心细胞类型的观点。我们的数据还表明，抗KS T细胞反应本质上是多克隆的，并非由靶向肿瘤相关抗原肽的T细胞克隆性扩增所致。
