Kagoshima M, Tomomatsu N, Iwahisa Y, Yamaguchi S, Matsuura M, Kawakami Y, Terasawa M
Research Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., Fukuoka, Japan.
Inflamm Res. 1997 Apr;46(4):147-53. doi: 10.1007/s000110050539.
Effects of Y-24180 on antigen-induced asthmatic responses were evaluated in actively sensitized guinea pigs and the effects were compared with those of several anti-asthmatic drugs.
Male Hartley guinea pigs were used.
Guinea pigs were actively sensitized with ovalbumin and were pretreated with pyrilamine Y-24180 was orally administered to the animals 3 h and others were 1 h before the antigen challenge.
The airway hyperresponsiveness was measured according to the method of Konzett and Rössler with some modifications. The immediate asthmatic response (IAR) and late asthmatic response (LAR) were measured by the oscillation method. Inflammatory cells infiltrated into the lungs were counted after the bronchoalveolar lavage.
Under oral administration before or after the challenge with antigen, Y-24180, OKY-046, and ONO-1078 suppressed the antigen-induced airway hyperresponsiveness. Moreover, Y-24180, ONO-1078, AA-2414, and theophylline suppressed both the IAR and LAR, but OKY-046 only suppressed the LAR. Among the test drugs, only Y-24180 and theophylline suppressed the antigen-induced accumulation of eosinophils in the bronchoalveolar lavage fluid.
The data indicate practical participation of PAF in the development of antigen-induced asthmatic responses in animals, and usefulness of Y-24180 in the clinical treatment of asthma as well as other anti-asthmatic drugs.
在主动致敏的豚鼠中评估Y - 24180对抗原诱导的哮喘反应的影响，并将其与几种抗哮喘药物的效果进行比较。
使用雄性Hartley豚鼠。
豚鼠用卵清蛋白主动致敏，并预先用吡苄明处理。Y - 24180在抗原激发前3小时口服给予动物，其他药物在抗原激发前1小时给予。
根据Konzett和Rössler的方法并稍作修改来测量气道高反应性。通过振荡法测量速发型哮喘反应（IAR）和迟发型哮喘反应（LAR）。支气管肺泡灌洗后对浸润到肺中的炎性细胞进行计数。
在抗原激发前后口服给药时，Y - 24180、OKY - 046和ONO - 1078抑制了抗原诱导的气道高反应性。此外，Y - 24180、ONO - 1078、AA - 2414和茶碱抑制了IAR和LAR，但OKY - 046仅抑制了LAR。在受试药物中，只有Y - 24180和茶碱抑制了抗原诱导的支气管肺泡灌洗液中嗜酸性粒细胞的积聚。
数据表明血小板活化因子（PAF）实际参与了动物抗原诱导的哮喘反应的发生发展，以及Y - 24180与其他抗哮喘药物在哮喘临床治疗中的有效性。
