Raczyńska A, Jarczyk G, Jarczyk J, Jedrzjczyk W
Katedry i Kliniki Chirurgii Ogólnej i Gastroenterologii A.M. w Bydgoszczy.
Pol Merkur Lekarski. 1996 Sep;1(3):165-8.
The present study was carried out to value the histological method of detection of Helicobacter pylori in gastric mucosa and to analyse the pathological changes of the Helicobacter pylori-positive patients with duodenal and gastric ulcer and with chronic gastritis. The studies population consisted of 150 patients, 76 F and 74 M. The samples were taken during gastroscopy from the prepyloric region and gastric body. After routine histological procedure they were stained HE and by Giemsa method for detection of H.pylori. The latter was identified in 56.6% of of the patients with duodenal ulcer, in 54.8% of those with gastric ulcer, in 17.6% of the patients after partial gastrectomy and in 46.9% of patients suffering from chronic gastritis. The increased density of H.pylori (third grade) was detected in the cases with clinical diagnosis of duodenal and gastric ulcer and erythematous endoscopic gastritis and was correlated with the histological picture of chronic active gastritis, which was characterized by diffuse mixed cellular exudation, lesion of the superficial epithelial, mucosal erosions and by foveolar hyperplasia. We suggest that this morphological picture corresponded to the active phase of Helicobacter infection. We conclude, that the histological method by Giemsas staining is effective especially in the acute phase of H.pylori infection and represents high diagnostic value and may be used in monitoring during the antibacterial (anti H.pylori) treatment. There is the correlation between massive density of H.pylori observed in gastric mucosa and chronic active gastritis.
本研究旨在评估胃黏膜中检测幽门螺杆菌的组织学方法，并分析幽门螺杆菌阳性的十二指肠溃疡、胃溃疡及慢性胃炎患者的病理变化。研究人群包括150名患者，其中女性76名，男性74名。样本在胃镜检查时取自幽门前区和胃体。经过常规组织学处理后，样本进行苏木精-伊红（HE）染色和吉姆萨染色以检测幽门螺杆菌。结果显示，十二指肠溃疡患者中幽门螺杆菌的检出率为56.6%，胃溃疡患者中为54.8%，部分胃切除术后患者中为17.6%，慢性胃炎患者中为46.9%。在临床诊断为十二指肠溃疡、胃溃疡及内镜下红斑性胃炎的病例中检测到幽门螺杆菌密度增加（三级），且与慢性活动性胃炎的组织学表现相关，其特征为弥漫性混合细胞渗出、浅表上皮损伤、黏膜糜烂及小凹增生。我们认为这种形态学表现对应于幽门螺杆菌感染的活跃期。我们得出结论，吉姆萨染色的组织学方法尤其在幽门螺杆菌感染的急性期有效，具有较高的诊断价值，可用于抗菌（抗幽门螺杆菌）治疗期间的监测。胃黏膜中观察到的幽门螺杆菌大量密度与慢性活动性胃炎之间存在相关性。
