采用捕获酶联免疫吸附测定法检测蚊子唾液特异性IgE抗体。
Detection of mosquito saliva-specific IgE antibodies by capture ELISA.
作者信息
Brummer-Korvenkontio H, Palosuo K, Palosuo T, Brummer-Korvenkontio M, Leinikki P, Reunala T
机构信息
National Public Health Institute, Helsinki, Finland.
出版信息
Allergy. 1997 Mar;52(3):342-5. doi: 10.1111/j.1398-9995.1997.tb01002.x.
We developed an IgE-capture ELISA and measured mosquito saliva-specific IgE antibodies in 27 children sensitive to mosquito bites. Children with large 15-min bite wheals had significantly higher (P < 0.0005) mosquito saliva-specific IgE levels than children with small wheals. In the latter group, the saliva-specific IgE level was significantly higher (P = 0.031) than the levels of six infants never exposed to mosquitoes. A positive correlation (r = 0.65; P = 0.0002) was found between the size of the 15-min wheal and the mosquito saliva-specific IgE antibody levels. These results further support the role of mosquito saliva-specific IgE antibodies in the pathogenesis of mosquito-bite whealing. Compared to immunoblotting, IgE-capture ELISA provides a quantitative method to measure mosquito saliva-specific IgE antibodies.
我们开发了一种IgE捕获酶联免疫吸附测定法，并检测了27名对蚊虫叮咬敏感的儿童体内的蚊虫唾液特异性IgE抗体。15分钟内叮咬风团大的儿童，其蚊虫唾液特异性IgE水平显著高于（P < 0.0005）风团小的儿童。在后一组中，唾液特异性IgE水平显著高于（P = 0.031）6名从未接触过蚊虫的婴儿。发现15分钟风团大小与蚊虫唾液特异性IgE抗体水平之间存在正相关（r = 0.65；P = 0.0002）。这些结果进一步支持了蚊虫唾液特异性IgE抗体在蚊虫叮咬风团发病机制中的作用。与免疫印迹法相比，IgE捕获酶联免疫吸附测定法提供了一种定量检测蚊虫唾液特异性IgE抗体的方法。
Zotero 插件